Workflow for T3E identification in RSSC strains and main outputs of the analysis. A. Flowchart for identification and manual annotation of T3E genes in the RSSC. B. T3E statistics for each of the curated strains in this study. 1. Number of T3E genes with a potential frameshift mutation within the coding sequence. 2. Number of annotated pseudogenes (incomplete or disrupted coding frames). 3. Number of hypothetical (candidate) T3E genes. 4. Total of estimated number of T3E genes (= number of T3E + frameshifted T3E + hypothetical T3E). 5. Not determined since the genome sequence of strain RS1000 is not available.