Dichaete and neuroblast segregation. A-C) Dichaete binding profiles at pros, mira and grim-rpr. Upper two blue plots represent 25% and 1% FDR DamID binding intervals; D_Dam represents the normalised window score of the triplicated DamID experiment; Core (red) represents the regions defined as core binding intervals overlapping DamID and ChIP data. The black regions represent gene models and the genome coordinates. D-G) anti-Prospero staining in Dichaete mutant embryos, all ventral view with anterior to the top. D) wild type stage 10. E) Dr72/Df(3 L)Gs1-a stage 10. F) wild type stage 11. G) Dr72/Df(3 L)Gs1-a stage 11. H-J) Anti-Prospero staining in embryos expressing dominant negative Dichaete constructs, images show the right half of two thoracic and 1 abdominal segment (T2, T3, A1). H) wild type, stage 12. I) prosGAL4, UAS-DΔHMG stage 12. J) prosGAL4, UAS-DEnRep stage 12. K and L) Anti-Pros staining in embryos expressing dominant negative mouse Sox2, ventral views of 2 abdominal segments from late stage 11 embryos. K) wild type. L) prosGAL4, UAS-mouseSox2EnRep.