Figure 1
From: De novo copy number variations in cloned dogs from the same nuclear donor
Whole-genome plot of CNVs and qPCR validation. (A) Whole-genome plot of CNVs identified between Labrador retriever and Boxer genomes. Nine CNV regions (7 CNV-Gs, red arrow; 2 CNV-Ls, green arrow) were randomly selected for qPCR validation. The X-axis represents individual chromosomes and the Y-axis represents signal intensity ratios (Labrador retriever/Boxer) on a log2 scale. (B) Log2ratio plot around the nine selected CNV regions. Black bars represent primer position for qPCR validation. The X-axis represents genomic position (Mb) and the Y-axis represents signal intensity ratios (Labrador retriever/Boxer) on log2 scale. (C) Three primer sets located in the CNV-L regions detected copy loss, and the other eight primer sets located in the CNV-G regions detected copy gain. The red and green line represent detection criterion for copy gain and copy loss, respectively. The Y-axis represents fold change based on the Boxer as the calibrator.