Table 3 Functional classification of transcripts coding for putative secreted proteins in female Psorophora albipes salivary glands

Enzymes

  Glycosidases

7

681,837

9.05

  5′ nucleotides/Apyrase family

6

41,550

0.55

  Serine proteases

5

40,574

0.54

  Cathepsins

3

36,397

0.48

  Serine-type carboxypeptidases

2

20,779

0.28

  Mosquito lipases

1

9,608

0.13

  Alkaline phosphatases

2

8,087

0.11

  Adenosine deaminase family

1

6,885

0.09

  Endonucleases

4

6,281

0.08

  Ribonucleases

3

2,417

0.03

  Destabilase family

1

842

0.01

  Hyaluronidases

1

372

0.00

Ubiquitous protease inhibitor domains

  Serpin family

15

169,094

2.24

  Kazal domain-containing peptides

13

46,712

0.62

  TIL domain family found in mosquitoes

8

38,268

0.51

  Metalloproteinase inhibitors

1

2,029

0.03

  Schistocerca protease inhibitor

1

2,023

0.03

  Cystatins—may be housekeeping

1

175

0.00

Immunity-related proteins

  Fred/Ficolin domain-containing proteins

9

65,681

0.87

  Lysozymes

7

59,845

0.79

  C type lectins

7

58,303

0.77

  Peptidoglycan recognition domains

7

16,505

0.22

  Gram-negative binding proteins

1

5,968

0.08

  ML domains

3

4,480

0.06

  Gambicin

2

4,360

0.06

  Leucine-rich proteins

1

1,249

0.02

  Cecropins

1

277

0.00

  Defensin

1

275

0.00

  Galectin—maybe housekeeping

1

173

0.00

Mucins

  Mucin I mosquito family

22

1,866,771

24.77

  gSG5 mucin protein family

8

60,819

0.81

  Aedes-specific mucins

7

30,550

0.41

  Other mucins

8

24,225

0.32

  Virus-induced mucins

1

11,007

0.15

  SG3 mucin family

2

2,197

0.03

  Peritrophin/chitin binding

3

1,587

0.02

  Simulium mucins

3

1,320

0.02

OBP superfamily

  Long-D7 mosquito family

26

297,507

3.95

  Culicine short-D7 proteins

23

156,429

2.08

  Salivary mosquito OBP

2

1,028

0.01

Yellow Phlebotomine family

2

7,763

0.10

Ubiquitous protein families existing outside Nematocera, function unknown

  Antigen-5 family

13

257,852

3.42

  Aedes hypothetical secreted conserved proteins

3

5,605

0.07

  12- to 14-kDa mosquito family similar to Drosophila proteins

4

3,609

0.05

  15- to 17-kDa insect family

1

816

0.01

  Culex/Drosophila WAP subfamily

1

283

0.00

Protein families exclusive of bloodsucking Nematocera

  30 kDa/Aegyptin family - Mosquitoes and black flies

5

197,279

2.62

  Canonical

8

109,171

1.45

  Mucin II mosquito family

2

9,705

0.13

Protein families specific to mosquitoes

  HHH peptide family

18

576,548

7.65

  30.5-kDa protein

4

327,913

4.35

  hyp8.2 Culicine family

6

98,361

1.30

  9.7-kDa family

17

63,969

0.85

  Aedes W-rich peptides

1

51,783

0.69

  Aedes 6.5–8.5 protein family

3

43,900

0.58

  Aedes 62-kDa family

6

41,700

0.55

  Basic tail mosquito family

6

32,687

0.43

  34-kDa Aedes family

8

30,082

0.40

  Aedes/Anopheles darlingi 14–15 family

6

25,350

0.34

  GQ-rich Culicine family

3

8,203

0.11

  23.5 kDa Culicine family

4

8,099

0.11

  gSG8 family

1

3,237

0.04

  Hyp6.2 family

3

2,291

0.03

  Culex WRP/16-kDa family

2

2,138

0.03

  Salivary protein 16 family

2

1,778

0.02

  HHH family 2

3

1,432

0.02

  Anopheline SG1 family

1

273

0.00

Protein families specific to black flies

  Simulium disintegrin similar to phenoloxidase inhibitor peptides

1

15,887

0.21

  H-rich, acidic proteins of Simulium

1

861

0.01

Salivary-orphan proteins of conserved secreted families

9

11,269

0.15

Deorphanized proteins

  Similar to OT-19—contains HH repeats

16

772,288

10.25

  Aedes 5-kDa family

7

125,986

1.67

  Aedes 7-kDa family

4

2,378

0.03

Families not reported on Nematocera - sialome review

  Lipocalins

5

23,805

0.32

  SGS family

4

4,869

0.06

Novel families

  Pso 4.7 kDa

6

47,608

0.63

  Pso 4.01 kDa ultrashort-D7 family

3

11,185

0.15

  Pso 4.2 kDa

12

4,201

0.06

  Pso 6.3 kDa

3

3,398

0.05

  Pso 12 kDa

3

1,289

0.02

  Pso 12.8 kDa—novel mosquito peptide family

2

1,217

0.02

  Pso 20.44 kDa—unique to Culicine

1

290

0.00

  Pso 4.69 kDa—unique to Aedes

1

103

0.00

Other putative secreted proteins

371

814,858

10.81

Total secreted

802

7,537,805