Identification of two putative reference genes from grapevine suitable for gene expression analysis in berry and related tissues derived from RNA-Seq data

Table 4 Candidate reference gene ranking according to their CV

Genoscope ID	Total reads	Mean^¥	SD	CV ^{^}	CHR	Product
GSVIVG01036166001*	80103	1704	791	0,46	chr6	Vacuolar protein sorting-associated protein 4
GSVIVG01013003001*	57177	1217	571	0,47	chr2	26S proteasome non-ATPase regulatory subunit 13
GSVIVG01027659001*	63133	1343	635	0,47	chr15	Unkown protein function
GSVIVG01025947001*	64396	1370	657	0,48	chr18	Protein AIG1
GSVIVG01035814001*	79018	1681	818	0,49	chr4	Unkown protein function
GSVIVG01038268001*	162669	3461	1689	0,49	chr5	Rab GDP dissociation inhibitor alpha
GSVIVG01008708001*	90480	1925	941	0,49	chr18	T-complex protein 1 subunit beta
GSVIVG01028520001*	96994	2064	1009	0,49	chr7	26S protease regulatory subunit 4 homolog
GSVIVG01012792001^ǂ	56443	1201	588	0,49	chr18	Putative peptidase
GSVIVG01031067001	67287	1432	705	0,49	chr14	T-complex protein 1 subunit zeta
GSVIVG01033771001	83853	1784	883	0,49	chr8	Splicing factor U2af small subunit A
GSVIVG01033172001	78058	1661	822	0,50	chr4	Serine/Arginine-rich splicing factor 7
GSVIVG01016731001	69545	1480	734	0,50	chr9	Proteasome subunit alpha type-6
GSVIVG01028854001	82587	1757	875	0,50	chr16	40S ribosomal protein S10-1
GSVIVG01033442001*	63350	1348	673	0,50	chr8	Carbon catabolite repressor protein 4 homolog 2
GSVIVG01037814001*	70685	1504	754	0,50	chr3	Unkown protein function
GSVIVG01015062001^ǂ	59049	1256	637	0,51	chr11	Aldehyde dehydrogenase family 7 member A1
GSVIVG01030215001*	155807	3315	1682	0,51	chr8	Proactivator polypeptide-like 1
GSVIVG01016593001*	101022	2149	1091	0,51	chr13	Actin-depolymerizing factor 2

SD standard deviation; CV coefficient of variation; CHR chromosome location for each gene.
*Genes studied in this work; ^ǂgenes that showed double amplicon.
^¥Threshold mean 1208.2 (percentile 97.5%).
^{^}Threshold coefficient of variation 0.513 (percentile 2.5%).

ISSN: 1471-2164