Amplification plate layout. Plate map for simultaneous amplification of eleven samples in a 96-well plate. Samples are organized by columns (Sample 1 in column 1, Sample 2 in column 2, etc.), and each row represents one of the eight mtGenome amplicons (Amplicon 1 in row 1, Amplicon 2 in row 2, etc.). Negative controls for each target fragment are amplified in column 12.