Sequencing plate maps for high-throughput processing. 96-well plate layouts for sequencing eleven complete mtGenomes in two sets (forward, A; and reverse, B), for a total of sixteen sequencing plates per eleven mtGenomes. The eleven different samples are indicated by Ind1, Ind2, etc. and are color-coded, and the primer for each plate well is listed. Empty wells are noted. These plate layouts represent one strategy for high-throughput sequencing, and were specifically designed for efficient pipetting on our laboratory’s liquid handling instruments (MICROLAB® STARlet and STARplus, Hamilton Robotics). A different layout may be more appropriate/more efficient with other instrumentation.