Figure 5From: A high-throughput Sanger strategy for human mitochondrial genome sequencingAmplification sensitivity with positive control DNA. Results from duplicate amplifications of all eight mtGenome fragments with ten input levels of positive control DNA (9947A). Amplification product concentrations of the target fragments were measured by automated injection on a QIAxcel Advanced capillary electrophoresis instrument (QIAGEN Inc.), and were normalized to ng/μL per 1000 bp (given the variable sizes of the eight target fragments). The wide range of product concentrations represented in the long “whiskers” reflects the differing amplification efficiencies of the eight primer pairs; however, PCR product was produced for all regions down to 10 pg input DNA.Back to article page