Figure 8From: A high-throughput Sanger strategy for human mitochondrial genome sequencingSequencing artifacts. A) Screen captures of forward sequences aligned in Sequencher (Gene Codes Corporation) showing compression and unusual peak morphology around nucleotide position 7600. B) When the same primer (F7366) was used without the addition of dGTP BigDye® (Life Technologies, Applied Biosystems), the sequencing artifacts were no longer apparent.Back to article page