A high-throughput Sanger strategy for human mitochondrial genome sequencing

Lyons, Elizabeth A; Scheible, Melissa K; Sturk-Andreaggi, Kimberly; Irwin, Jodi A; Just, Rebecca S

doi:10.1186/1471-2164-14-881

BMC Genomics

Table 2 Sequencing primers

From: A high-throughput Sanger strategy for human mitochondrial genome sequencing

Amplicon number	Primer name	Sequence	Source
1	F402	ATCTTTTGGCGGTATGCACTTT	New
	F619	TTAGACGGGCTCACATCACC	[19]
	R878	CCAACCCTGGGGTTAGTATAGC	New
	F900	CGGTCACACGATTAACCCAAG	New
	F1135	CCAGAACACTACGAGCCACA	New
	R1136	GGCGAGCAGTTTTGTTGATT	New
	F1320	GACGTTAGGTCAAGGTGTAGCC	New
	R1365	TAGCCCATTTCTTGCCACCT	New
	F1657	CTTGACCGCTCTGAGCTAAAC	[16, 17]
	R1769	GCCAGGTTTCAATTTCTATCG	[16, 17]
	R1924	AGGTAGCTCGTCTGGTTTCG	New
	F1983	TAGAGGCGACAAACCTACCG	New
	F2105	GAGGAACAGCTCTTTGGACAC	[16, 17]
	R2216	TGTTGAGCTTGAACGCTTTCTT	New
	F2333	GCATAAGCCTGCGTCAGAT	New
	R2439	ATGCCTGTGTTGGGTTGAC	New
	F2506	AACATCACCTCTAGCATCACCA	New
	R2818	GCCCCAACCGAAATTTTTAAT	New
2	F2625	CTGTATGAATGGCTCCACGAG	New
	F2932	GGGATAACAGCGCAATCCTAT	New
	R3006	ATGTCCTGATCCAACATCGAG	[16, 17]
	F3241	AGAGCCCGGTAATCGCATAA	New
	R3417	GGGGCCTTTGCGTAGTTGTA	New
	F3441	ACTACAACCCTTCGCTGACG	[16, 17]
	R3632	GAGGTGGCTAGAATAAATAGGAGGC	New
	F3635	GCCTAGCCGTTTACTCAATCC	[16, 17]
	R3825	TCAGAGGTGTTCTTGTGTTGTGAT	New
	F3890	GAACCCCCTTCGACCTTG	New
	R4162	TGAGTTGGTCGTAGCGGAATC	[16, 17]
	F4142	GATTCCGCTACGACCAACT	New
	F4392	CCCATCCTAAAGTAAGGTCAGC	[16, 17]
	R4479	GGGGATTAATTAGTACGGGAAGG	New
	R4676	GATTATGGATGCGGTTGCTT	New
	R4811	TCAGAAGTGAAAGGGGGCTAT	New
3	F4609	AAATAAACCCTCGTTCCACAGA	New
	F4925	CCTTCTCCTCACTCTCTCAATC	New
	R5034	ATCCTATGTGGGTAATTGAGGA	New
	F5150	CCTACTACTATCTCGCACCTGAA	New
	R5210	GGTGGATGGAATTAAGGGTGT	New
	F5318	CACCATCACCCTCCTTAACC	[16, 17]
	R5325	TGATGGTGGCTATGATGGTG	New
	R5681	GTGGGTTTAAGTCCCATTGGT	New
	F5664	AATGGGACTTAAACCCACAAA	New
	F5858	TTTACAGTCCAATGCTTCACTC	New
	R5799	TGCAAATTCGAAGAAGCAG	New
	R5994	TGCCTAGGACTCCAGCTCAT	[19]
	F6032	GCCAGGCAACCTTCTAGGTA	New
	F6318	CCTGGAGCCTCCGTAGACCT	New
	R6444	TTTGGTATTGGGTTATGGCAG	New
	F6496	CTCTCCCAGTCCTAGCTGCTG	New
	R6899	CATATTGCTTCCGTGGAGTGTG	New
4	F6636	ATTCTTATCCTACCAGGCTTCG	New
	F7075	GTATGGGGATAAGGGGTGTA	[16, 17]
	R7248	TGGTGTATGCATCGGGGTAGT	New
	F7366	CCTCCATAAACCTGGAGTGA	New
	R7489	TGGCTTGAAACCAGCTTTG	[19]
	F7527	GAAAAACCATTTCATAACTTTGTCA	New
	R7766	TTTCCTGAGCGTCTGAGATGT	New
	F7821	CATCCCTACGCATCCTTTACAT	New
	F8129	ACCACTTTCACCGCTACACG	New
	R8141	CGGTGAAAGTGGTTTGGTTTA	New
	F8355	TTTACAGTGAAATGCCCCAAC	New
	R8378	TTAGTTGGGGCATTTCACTGT	New
	R8640	GATGAGATATTTGGAGGTGGG	New
	F8668	TGACTAATCAAACTAACCTCAAAACA	New
	F8717	AAGGACGAACCTGATCTCTTATACT	New
	R8949	TAGTATGGGGATAAGGGGTGTA	New
	R9031	GGTGGCCTGCAGTAATGTTAG	New
5	F8940	CCCCATACTAGTTATTATCGAAACC	New
	F9272	CTCAGCCCTCCTAATGACCTC	New
	R9376	CATTGGTATATGGTTAGTGTGTTGG	New
	F9483	TTCTTCGCAGGATTTTTCTGA	New
	R9611	GGATGTGTTTAGGAGTGGGACT	New
	R9853	GTGAGGAAAGTTGAGCCAATAA	New
	F9832	TTATTGGCTCAACTTTCCTCAC	New
	R10171	TAGAAAAATCCACCCCTTACGA	New
	F10267	CCCTCCTTTTACCCCTACCAT	New
	R10294	AGGGCTCATGGTAGGGGTAA	New
	F10419	AACAAAACGAATGATTTCGACTC	New
	F10689	GGCCTAGCCCTACTAGTCTCAA	New
	R10715	CGTAGTCTAGGCCATATGTGTTG	[19]
	R10942	TAGGGGGTCGGAGGAAAAG	New
	F10950	CCCTCCTAATACTAACTACCTGACTC	New
	R11166	CATCGGGTGATGATAGCCAAG	[16, 17]
	R11428	GGCTTCGACATGGGCTTT	New
6	F11319	CAAACTCCTGAGCCAACAACTT	New
	F11760	ACGAACGCACTCACAGTCG	[16, 17]
	R11768	TGCGTTCGTAGTTTGAGTTTG	New
	R11804	GAAGTCCTTGAGAGAGGATTATGA	New
	F11964	TCACAGCCCTATACTCCCTCT	New
	R12089	TGGGGGATAGGTGTATGAACA	New
	F12194	CCCCTTATTTACCGAGAAAGC	New
	R12302	GCCTAAGACCAATGGATAGCT	New
	F12452	TTGTCGCATCCACCTTTATT	New
	F12741	CAACCTATTCCAACTGTTCATCG	New
	R12766	AGCCGATGAACAGTTGGAATA	New
	R13025	TGGAGACCTAATTGGGCTGA	New
	F13203	AGTCTGCGCCCTTACACAAA	New
	R13390	TGTTAAGGTTGTGGATGATGGA	New
	R13559	GCTCAGGCGTTTGTGTATGAT	New
	F13628	CTAACAGGTCAACCTCGCTTC	New
	R13855	GGTAGTTGAGGTCTAGGGCTGTT	New
	R13924	GGTAGAATCCGAGTATGTTGGAG	New
7	F13835	CAGCCCTAGACCTCAACTACC	New
	F14058	CATCATCACCTCAACCCAAA	New
	R14118	TGGGAAGAAGAAAGAGAGGAAG	[16, 17]
	F14431	TGCCTCAGGATACTCCTCAAT	New
	R14448	GAGGAGTATCCTGAGGCATGG	New
	F14641	ACCCACACTCAACAGAAACAAA	New
	R14721	CGATGGTTTTTCATATCATTGG	New
	F14881	CACCACAGGACTATTCCTAGCC	New
	R14902	GGCTAGGAATAGTCCTGTGGTG	New
	F15190	CTTACTATCCGCCATCCCATA	New
	R15396	TTATCGGAATGGGAGGTGATTC	[16, 17]
	F15500	GACCCAGACAATTATACCCTAGCC	New
	R15585	ATTGTGTAGGCGAATAGGAAATA	New
	F15699	GCCCACTAAGCCAATCACTT	[19]
	R15728	GGAGTCAATAAAGTGATTGGCTTAG	New
	R16042	CTGCTTCCCCATGAAAGAAC	[19]
8	F15971	TTAACTCCACCATTAGCACC	[13]
	F16190	CCCCATGCTTACAAGCAAGT	[13]
	F155	TATTTATCGCACCTACGTTC	[13]
	F314	CCGCTTCTGGCCACAGCACT	[13]
	R16410	GAGGATGGTGGTCAAGGGA	[13]
	R285	GTTATGATGTCTGTGTGGAA	[13]
	R484	TGAGATTAGTAGTATGGGAG	[13]
	R599	TTGAGGAGGTAAGCTACATA	[13]

Sequencing primers for the complete mtGenome, with sources (new or previously published). All primers for Amplicon 8, which covers the mtDNA CR, were adopted from [13]; these are used in duplicate to produce a total of sixteen sequences for the CR. Most amplification primers are also utilized as sequencing primers (see Table 1).

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ISSN: 1471-2164

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