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Table 2 Summary of the UV-8b physical maps autobuilt from assembly of the 2,290 BAC clones at different stringencies

From: A BAC based physical map and genome survey of the rice false smut fungus Villosiclava virens

Cutoff Contigs Avr contig length (kb)1 Longest contig (kb)1 Physical length (Mb)1 Q contigs/ Q clones (%)2 No. contigs containing different clone numbers Singletons (%)3
>50 50-26 25-10 9-3 =2
10-30 314 184 287 58.2 0/0 (0.00) 0 0 18 180 116 991 (43.3)
10-25 305 196 376 60.0 7/7 (0.31) 0 0 43 168 94 653 (28.5)
10-20 255 217 774 55.5 36/49 (2.14) 0 4 57 136 58 428 (18.7)
10-18 231 230 774 53.4 41/63 (2.75) 0 9 61 112 49 357 (15.6)
10-15 196 261 774 51.3 63/111 (4.85) 1 15 61 78 41 255 (11.1)
10-12 161 302 1032 48.7 74/164 (7.16) 3 20 53 62 23 177 (7.7)
10-10 142 335 1585 47.7 77/174 (7.60) 5 20 51 48 18 160 (7.0)
10-08 135 340 1585 46.0 80/204 (8.91) 5 22 50 44 14 134 (5.9)
Phase IA 4 194 263 774 51.2 63/111 (4.85) 1 15 61 76 41 255 (11.1)
  1. 1Each fingerprint band was estimated to be 1.13 kb based on the average insert size 140 kb and an average 124 bands per clone.
  2. 2The percentage of Q clones to total clones 2290.
  3. 3The percentage of single clones to total clones 2290.
  4. 4The cutoff value of 10-15 was chosen, then contig101was end-merged to contig76, and contig193 was end-merged to contig1 by “End to End” function at cutoff 10-12, respectively.