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Table 4 Oligonucleotides used as adapters and primers in this study

From: Comprehensive analysis of the Corynebacterium glutamicum transcriptome using an improved RNAseq technique

Name

Sequence (5′-3’)

RNA adapter

CCCUACACGACGCUCUUCCGAUCGAG

stem-loop DNA adapter

AGATCGGAAGAGAGACGTGTGCTCTTCCGATCTNNNNNNN

amplification primer 1

AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCGAG

amplification primer 2

CAAGCAGAAGACGGCATACGAGATCGTGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT

  1. All oligonucleotides used in this work were synthesized at Metabion International AG (Martinsried, Germany).
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BMC Genomics

ISSN: 1471-2164

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