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Table 1 KIR haplotyping methodology – primary and secondary SNP positions

From: Recombinant structures expand and contract inter and intragenic diversification at the KIR locus

Amplicona

Exon

Genes amplifiedb

Primary validationc

Secondary confirmationd

2DL2-001

5

2DL2, 2DL3, 2DS2, 2DP1, (3DL2)

2DL2-93-T

2DP1-137-T/2DS2-209-T

2DL3-006

7/8

2DL3, 2DS3/5

2DL3-136-G; -616-T

2DS35-59-A

2DL5

3

2DL1/4/5 3DP1

2DL5-170-G; -174-A

2DL5-58-G; -79-T; -103-C; -157-A

2DP1-002

3

2DP1

2DP1-128-G; -130-A

 

2DS2-002

4

2DL1/2/3, 2DS2, 3DP1

2DS2-89-A

2DL1-60-A; -117-A;-154-A

2DS35-005

4

2DS1, 2DS3/5, 3DP1, (2DL1)

2DS35-247-T; -251-G; -403-A; -404-T2DS3-446-G; -507-T2DS5-376-C

2DS1-446-A

3DL1-002

4

3DL1, 3DS1

3DL1-85-T3DS1-85-G

 

3DL1-005

4

3DL1, 3DS1

3DL1-157-C; -167-T3DS1-157-T; -167-G

 

KIR-2DL2-N1

4

2DL2/3, 2DS2, 2DP1

2DL2-126-G

2DS2-213-A2DP1-217-C

KIR-2DS2-N1

5

2DL2/3 2DP1 2DS2

2DS2-92-T

2DL2-179-T

2DS1-4-002

4

2DS1, 2DL1, 2DS4 (2DS35)

2DS1-257-AG2DL1-257-C2DS4-86-T; -95-A

2DS4-76-T; -86-T; -138-G; -187-A; -95-A; -179-A; -197-A; -225-T; -246-T; -345-G

2DS1-004

4

2DS1, 2DL1, (2DS4)

2DS1-80-AG2DL1-80-C

2DL1-139-G2DS1-139-T

3DL1-3DS1-01

4

3DL1, 3DS1, 3DL2

multiple SNPse

 

3DL1-3DS1-05

4

3DL1, 3DS1, 3DL2

multiple SNPse

 
  1. a see supplementary Additional file1: Table S3 for primer sequences.
  2. b loci listed in parenthesis indicate secondary PCR products amplified only in the absence of the target locus.
  3. c The position(s) of SNP(s) within the amplicon uniquely identifying the target locus.
  4. d The position(s) of SNP(s) which identify other loci amplified by that assay.
  5. e multiple SNP positions indicate presence or absence of 3DL1/S1.