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Table 6 Microarray hybridization design

From: The early phase transcriptome of bovine monocyte-derived macrophages infected with Staphylococcus aureus in vitro

 

No of arrays/dye-swaps

Individual

A

B

C

D

E

F

Total

2 h incubation

2

1

1

1

1

2

8

6 h incubation

2

2

1

2

2

2

11

  1. Uninfected control samples were used as references, i.e. control and Staphylococcus aureus infected samples from one individual at one time point were hybridized to one array. Dye-swap design was used, i.e. uninfected control samples were labeled with Cy5 and Staphylococcus aureus infected samples were labeled with Cy3, and subsequently swap labeling was applied.