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Figure 1 | BMC Genomics

Figure 1

From: OnPLS integration of transcriptomic, proteomic and metabolomic data shows multi-level oxidative stress responses in the cambium of transgenic hipI- superoxide dismutase Populus plants

Figure 1

Schematic flowchart of the integrated profiling strategy applied in this study. In the first step, transcriptomic, proteomic and metabolomic data were collected individually from the cambial region of Populus WT and transgenic plants. In the second step, the three omic datasets acquired were integrated by OnPLS to identify the joint variation in them (initially applying OnPLS modeling to all variables, and subsequently to targeted variables). Finally, the OnPLS model from the second step was visualized by Mapman and KEGG to explore the pathways (genes-proteins-metabolites) affected in the transgenics, and deepen the interpretation of their oxidative stress responses.

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