Figure 7

Correlation between gene expression and levels of different ITs in a validation population using sparse canonical correlation. The correlation between the blood transcriptome profiles of 74 animals and the corresponding levels of IL2 and IL10 production, PHAG activity and CD4^-/CD8⁺ cell counts was measured. For each of these ITs, the top 50 genes that were differentially expressed between the high and low groups were selected. A) ITs and genes are represented through their projections onto a circle of radius 1 centred at the origin called correlation circle. Strongly associated variables are projected in the same direction from the origin. The greater the distance from the origin, the stronger the association. Only association scores greater than 0.50 with at least one of the ITs are displayed. B) ITs and genes are represented through a network. The network is displayed graphically as nodes (genes and ITs) and edges (the biological relationship between nodes). The edge colour intensity indicates the expression of the association: red = positive and blue = negative. Node shape indicates whether it is a gene (round) or an IT (square). The score of the association was indicated under each edge. Only pairwise associations with scores greater than 0.50 were projected. The symbol of genes identified as candidate biomarkers is indicated in blue in figure A and red in figure B.