The peripheral blood transcriptome reflects variations in immunity traits in swine: towards the identification of biomarkers

Table 1 Basic statistics describing the difference between high and low groups for in vitro production of IL2, IL10, IFNγ and TNFα, phagocytosis activity, and in vivo CD4 ^- /CD8 ⁺ and TCRγδ + cell counts, and anti- Mycoplasma antibody levels

Type of trait	Trait symbol	Group	Animal number	Mean	SD⁵	CV⁶(%)	p-value
In vitro stimulation	IL2¹	Low	11	-4.66	0.084	1.80	<0.0001
		High	10	3.20	0.243	7.59
	IL10¹	Low	10	-4.80	0.240	5.00	<0.0001
		High	10	1.71	0.056	3.27
	IFNγ¹	Low	11	-6.65	1.649	24.8	<0.0001
		High	7	2.63	0.174	6.06
	TNFα¹	Low	9	-2.41	0.496	20.61	<0.0001
		High	8	1.48	0.207	4.46
	PHAG²	Low	11	4.53	0.326	7.06	<0.0001
		High	9	9.04	0.316	3.43
In vivo	CD4^-/CD8⁺³	Low	5	-3.52	0.697	19.80	0.0002
		High	3	2.96	0.622	7.43
	TCR γδ⁺³	Low	8	-3.59	0.338	9.41	<0.0001
		High	9	4.49	0.338	7.52
	IgG-Mh⁴	Low	10	-3.24	0.162	5.00	<0.0001
		High	10	4.12	0.776	18.83

Traits were all transformed using Box-Cox transformation, thus making the transformed data symmetric; ¹means were expressed as transformed pg of cytokine/mL of blood; ²phagocytosis activity was expressed as transformed percentages; ³leukocyte sub-populations were expressed as transformed cell counts/mL; ⁴total concentrations of immunoglobulin G against M. hyopneumoniae were expressed as transformed pg of IgG-Mh/mL of blood; ⁵standard deviation; ⁶coefficient of variation.

ISSN: 1471-2164