Effect of epigenetic drugs in P-glycoprotein expression in PCa cell lines after pharmacological treatment. (A) Protein gel blot analysis (1- Mock; 2–0.5 μM TSA; 3- 1μM DAC + 0.5 μM TSA). β-actin was used as loading control. (B) Relative levels of Pglycoprotein. Values of three independent experiments (average ± standard deviation) are represented as fold variation in comparison to Mock cells. Optical density of visible bands was determined and normalized with β-actin levels (**p < 0.01, ***p < 0.001; Oneway ANOVA, Dunnett’s Multiple Comparison Test).