Figure 6

Neuron-specific translational stop-codon read-through of the Platynereis FVa/EFLGa pNP gives rise to two types of neuropeptides. (A) Precursor scheme of the Platynereis FVa/EFLGa pNP with the signal peptide (blue), cleavage sites (red), stop codons (grey) and predicted FVa and EFLGa peptides (yellow) indicated. (B) Anterior view of 48 hpf Platynereis larva stained with antibodies against EFLGa (left) and FVa (right). (C) Anterior view of 72 hpf Platynereis larva stained with an antibody against EFLGa (left), an antibody against EFLGa blocked with synthetic EFLGa peptide (middle) and an FVa antibody (right). (D) Dorsal view of 72 hpf Platynereis larvae stained for EFLGa (left) and a close-up of the EFLGa expressing neuron pair (left). (E) Dorsal view of 72 hpf Platynereis larva stained for FVa (left) and a close-up scan of a FVa expressing neuron pair (left). All samples were counterstained with a mouse acetylated-tubulin antibody (white). Green asterisks label neurons where FVa and EFLGa peptides co-occur. Scale bars: 50 μm. Abbreviations: pt: prototroch; mt: metatroch; pat1: first paratroch; pat2: second paratroch; tt: telotroch.