Uncovering the genetic basis for early isogamete differentiation: a case study of Ectocarpus siliculosus

BMC Genomics

Table 2 Validation of SOLiD based gene expression profiles by Real-Time PCR

		Log₂(Average relative expression)^a
Gene name	Gene function	RT-PCR	SOLiD	Validation (yes/no)
Esi0102_0070	Arf1, ARF family GTPase	−1.93	−2.05	Y
Esi0067_0029	long chain acyl-coA synthetase	−4.19	0.49	N
Esi0069_0059	Mannuronan C-5-epimerase	−2.98	0.06	N
Esi0101_0018	Tubular mastigoneme-related protein	6.16	4.72	Y
Esi0104_0023	GPCR-like protein	4.72	2.22	Y
Esi0130_0068	PKD/REJ-like protein	0.62	2.22	Y
Esi0418_0017	MORN motif precursor	4.95	32.19	Y
Esi0123_0020	hypothetical protein	−7.15	−31.61	Y
Esi0161_0002	Metal ion transporter-like protein	3.50	−32.10	N
Esi0098_0063	hypothetical protein	−4.92	−31.61	Y

^aRelative expression is calculated as a ratio of expression levels in male/female gametes to indicate genes up- or downregulated in male gametes. A gene is considered differentially expressed if its relative expression is twofold or greater.

ISSN: 1471-2164