Figure 2

Relationship among nucleosome, RNAP II occupancy and the usage of polyA sites. (A-B) The nucleosome distribution and RNAP II occupancy near constitutive polyA sites of highly expressed and lowly expressed genes in CD4+ T cells (highly expressed genes: RPKM > 10, blue curve; lowly expressed genes: RPKM < 0.1, red curve). (C-D) The nucleosome distribution and RNAP II occupancy near the high-usage (blue curve) and low-usage (red curve) alternative polyA sites in expressed genes (RPKM > 1) in CD4+ T cells. A high-usage site has the lowest RUD in the gene, and a low-usage site has the highest RUD in the gene.