Combined ChIP-Seq and transcriptome analysis identifies AP-1/JunD as a primary regulator of oxidative stress and IL-1β synthesis in macrophages

Table 2 Microarray results of transcripts demonstrating greater than three-fold difference in expression between Jund siRNA and scrambled control siRNA transfected WKY BMDMs

Gene symbol	Gene name	Chr.	Fold change	FDR (%)	Gene Function
Basal condition
Klrb1a	killer cell lectin-like receptor subfamily B member 1B	4	9.0	<0.01	IL-1β synthesis [19]
Mt2A	metallothionein 2A	19	4.6	0.63	Oxidative stress [15]
Il1b	interleukin 1 beta	3	4.1	2.85	IL-1β synthesis
Cxcl9	chemokine (C-X-C motif) ligand 9	14	3.5	<0.01	Inflammation
Lcn2	lipocalin 2	3	3.3	<0.01	Oxidative stress [16]
Vcan	versican	2	3.3	<0.01	Oxidative stress [17]
Nlrp3	NLR family, pyrin domain containing 3	10	3.2	3.01	IL-1β synthesis [21]
D3ZIY9_RAT	uncharacterised protein	10	3.1	1.09	-
Dot1l	DOT1-like, histone H3 methyltransferase (S. cerevisiae)	7	-3.7	2.30	Inflammation
Hspb1	heat shock protein 1	12	-3.8	<0.01	Oxidative stress [18]
Prkca	protein kinase C, alpha	10	-4.1	1.83	Oxidative stress [19]
LPS stimulated condition
Klrb1a	killer cell lectin-like receptor subfamily B member 1B	4	4.6	<0.01	IL-1β synthesis [20]
Hspb1	heat shock protein 1	12	-4.0	<0.01	Oxidative stress [18]

Differentially expressed transcripts with greater than three-fold difference in expression (four technical replicates per strain) were identified at a <5% FDR threshold following 40,000 permutations. Fold changes are of control siRNA versus Jund siRNA expression. A positive fold change indicates higher expression in BMDMs transfected with scrambled control siRNA i.e. with a higher level of Jund expression compared to Jund siRNA. Abbreviations: Chr.; chromosome, FDR: false discovery rate.

ISSN: 1471-2164