Table 1 Expression levels of selected biomass-conversion enzyme-genes in the three sections of the fungus garden
Tag id | Enzyme function deduced from BLAST hits | CAZy familya | Top | Middle | Bottom | Test for equal level P | Fold up, bottom relative to top (95% CI) |
|---|---|---|---|---|---|---|---|
Mean tag count (95% CI) | |||||||
23641 | Endocellulase | GH5, CBM1 | 6 (0, 14) | 41 (0, 123) | 370 (150, 591) | 0.021 | 57.9 (25.21, 268.15) |
16339 | Endocellulase | GH5 | 96 (13, 178) | 28 (0, 60) | 140 (56, 224) | 0.025 | 1.5 (0.71, 3.98) |
10483 | Cellobiohydrolase (CBHI-I) | GH7, CBM1 | 14 (0, 28) | 56 (0, 158) | 524 (349, 698) | 2.2e-3 | 36.4 (20.10, 107.96) |
24891 | Cellobiohydrolase (CBHI-II) | GH7 | 449 (13, 885) | 51 (0, 137) | 1211 (911, 1512) | 2.2e-4 | 2.7 (1.53, 8.43) |
20092 | β-Glucosidase, intracellular | GH3 | 23 (1, 45) | 12 (0, 33) | 58 (38, 79) | 7.2e-3 | 2.6 (1.41, 7.54) |
2990 | Lytic polysaccharide monooxygenase | AA9 | 0 | 21 (0, 60) | 96 (53, 140) | 0 | NA |
323 | Xylanase | GH10, CBM1 | 7 (0, 21) | 92 (0, 274) | 669 (360, 977) | 8.0e-3 | 101.3 (35.68, 1089.35) |
7614 | Acetyl xylan esterase | CE1, CBM1 | 88 (38, 137) | 175 (0, 395) | 1168 (917, 1419) | 3.0e-4 | 13.3 (9.12, 22.92) |
18781 | Xyloglucanase | GH12 | 16 (1, 31) | 95 (70, 119) | 141 (0, 287) | 4.3e-4 | 8.8 (2.32, 29.17) |
29382 | Xyloglucanase | GH74, CBM1 | 85 (5, 164) | 41 (0, 100) | 540 (324, 756) | 2.0e-3 | 6.4 (3.48, 18.33) |
17822 | Pectinesterase | CE8 | 77 (0, 159) | 39 (0, 81) | 209 (128, 290) | 4.9e-3 | 2.7 (1.43, 10.17) |
24998 | Pectinesteraseb | CE8 | 529 (295, 763) | 435 (297, 572) | 698 (304, 1092) | 0.25 | 1.3 (0.75, 2.19) |
13889 | Polygalacturonaseb | GH28 | 403 (273, 533) | 386 (244, 527) | 686 (418, 954) | 0.075 | 1.7 (1.16, 2.43) |
16820 | Pectate lyaseb | PL1 | 34 (11, 56) | 19 (0, 57) | 189 (10, 368) | 0.11 | 5.6 (1.88, 12.58) |
25523 | Pectate lyase | PL1 | 222 (74, 370) | 146 (0, 381) | 704 (547, 860) | 8.5e-4 | 3.2 (2.06, 6.01) |
12708 | Pectate lyase | PL3 | 6 (0, 17) | 54 (0, 160) | 655 (398, 912) | 4.1e-3 | 116.9 (45.09, 1220.07) |
29370 | Rhamnogalacturonan lyase | PL4 | 208 (87, 329) | 259 (125, 392) | 757 (477, 1036) | 6.0e-3 | 3.6 (2.28, 6.45) |
17541 | Endo-1,5-α-L-arabinanase | GH43 | 141 (22, 260) | 81 (31, 131) | 204 (110, 297) | 0.043 | 1.4 (0.79, 3.63) |
17220 | Glucoamylase | GH15, CBM20 | 32 (1, 62) | 35 (0, 107) | 130 (113, 146) | 8.6e-4 | 4.1 (2.40, 11.80) |
26445 | α-Galactosidase | GH27 | 162 (79, 245) | 163 (93, 233) | 660 (578, 742) | 5.7e-6 | 4.1 (2.96, 6.40) |
10080 | Cutinase | - | 433 (0, 889) | 367 (156, 577) | 960 (743, 1177) | 2.4e-3 | 2.2 (1.24, 7.86) |
28797 | Cutinase | - | 211 (0, 444) | 242 (0, 555) | 468 (340, 596) | 0.067 | 2.0 (1.18, 9.25) |
14730 | Esterase/lipase | - | 2 (0, 7) | 31 (0, 88) | 357 (298, 416) | 9.9e-5 | 148.6 (65.90, 1290.24) |
1743 | Lipase | - | 144 (28, 259) | 595 (424, 765) | 718 (423, 1013) | 6.7e-4 | 5.0 (2.78, 12.01) |