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Table 1 Expression levels of selected biomass-conversion enzyme-genes in the three sections of the fungus garden

From: The fungal symbiont of Acromyrmex leaf-cutting ants expresses the full spectrum of genes to degrade cellulose and other plant cell wall polysaccharides

Tag id Enzyme function deduced from BLAST hits CAZy familya Top Middle Bottom Test for equal level P Fold up, bottom relative to top (95% CI)
Mean tag count (95% CI)
23641 Endocellulase GH5, CBM1 6 (0, 14) 41 (0, 123) 370 (150, 591) 0.021 57.9 (25.21, 268.15)
16339 Endocellulase GH5 96 (13, 178) 28 (0, 60) 140 (56, 224) 0.025 1.5 (0.71, 3.98)
10483 Cellobiohydrolase (CBHI-I) GH7, CBM1 14 (0, 28) 56 (0, 158) 524 (349, 698) 2.2e-3 36.4 (20.10, 107.96)
24891 Cellobiohydrolase (CBHI-II) GH7 449 (13, 885) 51 (0, 137) 1211 (911, 1512) 2.2e-4 2.7 (1.53, 8.43)
20092 β-Glucosidase, intracellular GH3 23 (1, 45) 12 (0, 33) 58 (38, 79) 7.2e-3 2.6 (1.41, 7.54)
2990 Lytic polysaccharide monooxygenase AA9 0 21 (0, 60) 96 (53, 140) 0 NA
323 Xylanase GH10, CBM1 7 (0, 21) 92 (0, 274) 669 (360, 977) 8.0e-3 101.3 (35.68, 1089.35)
7614 Acetyl xylan esterase CE1, CBM1 88 (38, 137) 175 (0, 395) 1168 (917, 1419) 3.0e-4 13.3 (9.12, 22.92)
18781 Xyloglucanase GH12 16 (1, 31) 95 (70, 119) 141 (0, 287) 4.3e-4 8.8 (2.32, 29.17)
29382 Xyloglucanase GH74, CBM1 85 (5, 164) 41 (0, 100) 540 (324, 756) 2.0e-3 6.4 (3.48, 18.33)
17822 Pectinesterase CE8 77 (0, 159) 39 (0, 81) 209 (128, 290) 4.9e-3 2.7 (1.43, 10.17)
24998 Pectinesteraseb CE8 529 (295, 763) 435 (297, 572) 698 (304, 1092) 0.25 1.3 (0.75, 2.19)
13889 Polygalacturonaseb GH28 403 (273, 533) 386 (244, 527) 686 (418, 954) 0.075 1.7 (1.16, 2.43)
16820 Pectate lyaseb PL1 34 (11, 56) 19 (0, 57) 189 (10, 368) 0.11 5.6 (1.88, 12.58)
25523 Pectate lyase PL1 222 (74, 370) 146 (0, 381) 704 (547, 860) 8.5e-4 3.2 (2.06, 6.01)
12708 Pectate lyase PL3 6 (0, 17) 54 (0, 160) 655 (398, 912) 4.1e-3 116.9 (45.09, 1220.07)
29370 Rhamnogalacturonan lyase PL4 208 (87, 329) 259 (125, 392) 757 (477, 1036) 6.0e-3 3.6 (2.28, 6.45)
17541 Endo-1,5-α-L-arabinanase GH43 141 (22, 260) 81 (31, 131) 204 (110, 297) 0.043 1.4 (0.79, 3.63)
17220 Glucoamylase GH15, CBM20 32 (1, 62) 35 (0, 107) 130 (113, 146) 8.6e-4 4.1 (2.40, 11.80)
26445 α-Galactosidase GH27 162 (79, 245) 163 (93, 233) 660 (578, 742) 5.7e-6 4.1 (2.96, 6.40)
10080 Cutinase - 433 (0, 889) 367 (156, 577) 960 (743, 1177) 2.4e-3 2.2 (1.24, 7.86)
28797 Cutinase - 211 (0, 444) 242 (0, 555) 468 (340, 596) 0.067 2.0 (1.18, 9.25)
14730 Esterase/lipase - 2 (0, 7) 31 (0, 88) 357 (298, 416) 9.9e-5 148.6 (65.90, 1290.24)
1743 Lipase - 144 (28, 259) 595 (424, 765) 718 (423, 1013) 6.7e-4 5.0 (2.78, 12.01)
  1. aCarbohydrate-Active Enzymes family [27]: GH, glycoside hydrolase; AA, auxiliary activity; CBM, carbohydrate-binding module; CE, carbohydrate esterase; PL, polysaccharide lyase.
  2. bIdentified by Schiøtt et al. [7] [GenBank:HQ174765-HQ174767].