Ultra-deep mutant spectrum profiling: improving sequencing accuracy using overlapping read pairs

BMC Genomics

Table 1 Sequence coverage for the three natural RNA viral samples and the two plasmid control samples

	Rabies Fox1	Rabies Fox2	BCV	BCV-Control	Rabies-Control
No. bases sequenced	10,905 bp	11,183 bp	13,434 bp	1,012 bp	1,128 bp
Raw/single read coverage	518,930	522,347	356,027	732,294	4,306,793
ORP (Q≥30) coverage	80,546	96,198	79,037	128,943	773,021
Singleton ends coverage	139,599	114,217	69,584	176,087	1,098,167

Mean coverage per base is shown for raw reads (single, unpaired reads), overlapped regions of read pairs and singleton ends portions of the read pairs. Coverage for overlapping read pairs (ORP Q≥30) are reported in the unit of pairs in this table, to reflect that the two strands in the pair report redundant information on one insert fragment.

ISSN: 1471-2164