Figure 3

Patterns of microRNA paralog emergence. a. Multiple sequence alignment for microRNA paralog family hsa-mir-1233. Red frame and arrow indicate the mature microRNA sequences and their orientations. The seed regions (2–8) are not highly conserved. b. cDNA evidence. Typical blast results show the alignment of duplicate (red lines) with expressed transcripts (pink lines) (Additional file6). The 82 bp fragment containing the mature microRNA sequence cannot align to mRNA or non-coding RNA sequences in the public database, which could be spliced out.