Figure 5

Increased NFI promoter association does not correlate with high predicted affinity. Average NFI (panel A) or CTCF (panel B) ChIP-Seq tag counts were calculated in windows of 100 bp for regions 10kb up- and down- stream of the RefSeq annotated transcription start sites (TSS). TSS were oriented and the broken arrows indicate the initiation site and the direction of transcription. Tag counts were normalized globally, as a fold increase over the genome-wide average tag count in a window of 100bp. NFI and CTCF predicted site counts were normalized similarly as a fold increase over the genome average site count in a window of 100 bp using the indicated weight matrix cut-off scores.