From: A unified approach for allele frequency estimation, SNP detection and association studies based on pooled sequencing data using EM algorithms
α= 0.05%
α= 0.1%
α= 0.5%
α= 1%
f
R E f ^ avg
R E f ^ em
0.1%
52.0
9.4
102.0
15.6
502.0
72.0
1000.0
146.0
0.5%
10.3
4.0
20.2
4.9
99.5
13.3
199.0
26.5
1%
5.0
3.3
10.0
3.7
49.2
5.7
98.3
9.5
5%
1.0
5.4
1.9
6.0
9.1
6.7
18.1
6.3