Root mean square fluctuations of CA. Root mean square fluctuations of CA as a function of residue number for all the simulations. Different colors represent different simulations trajectories of spin labeled vinculin tail protein. As may be seen, the pattern of fluctuations is similar in all cases, indicating that the MD results are yielding reliable information as to the dynamics of the system. The higher peaks represent the deviation in the loop regions and the lower values represent the fluctuations along the helices. The five helices of Vt are also shown in the upper part of the figure.