Expression analyses of glycolytic enzyme pyruvate kinase-muscle 2 (PKM2) in response to HHQ treatment in human breast cancer cell, MDA-MB-231 and MCF-7. The breast cancer cells were treated with 12.5 μM and 25 μM of HHQ for 48 h at 37 °C. Representative western blot hybridization signals for PKM2 [A] and the quantitation of the signals is shown in [B]. Immunofluorescent detection of PKM2 is shown in [C] and the relative intensity measurement of immunofluorescence is shown in [D]. Significant decrease in PKM2 expression was observed in HHQ treated cells. Data are representative of three independent experiments done in triplicates and expressed as mean ± S.E.M. ''*'' represents the statistical significant (p<0.05) difference between control and HHQ treated groups.