PPARγ dependent repression of glycolytic enzymes PGK1 and PKM2 by HHQ in human breast cancer cells, MDA-MB-231 and MCF-7. The breast cancer cells were pre-treated with a potent PPARγ antagonist, GW9962 (10 μM) for 4 h at 37 °C, followed by treatment with 12.5 μM and 25 μM of HHQ for 48 h at 37 °C. Representative western blot hybridization signals for PGK1 [A] and PKM2 [B] for both the breast cancer cell lines and the quantitation of the signals is shown in [C] and [D], respectively. GW9662 showed no effect of HHQ on expression of PGK1 and PKM2, thereby suggesting that the activation of PPARγ is required for repression of these enzymes. Data are representative of three independent experiments and expressed as mean ± S.E.M.