Figure 3

Differential expression of multiple cell migration-related genes in human thymic epithelial cells after ITGA6 gene knock-down. The figure shows heat map of gene expression for selected extracellular matrix and chemokine ligand and receptor genes. Differential expression was defined with PCR arrays, comparing ITGA6 siRNA treated TEC with control siRNA. Bayesian gene clustering was done with sample profiles in ITGA6 siRNA silenced TEC (n = 5) versus control siRNA-treated TEC (n = 5). The graphic displays dendrograms representing the 1D clusterization of genes (left) and samples (top) and the 2D map corresponding to the levels of standardized gene expression profiles (z-score). The green areas display a decreased expression of the analyzed transcripts of the ITGA6 siRNA group, in comparison to the expression in the control siRNA group (down-regulated) and the red areas display an increased expression (up-regulated). Red dotted lines in dendrograms (left-left and top-left) indicate statistic significant weak unions, discouraged by the Bayesian clustering analysis. Values represented in the dendrogram branches correspond to log-odds of the union of corresponding branches. Colorkey indicate gene z-scores on "Control siRNA" and ITGA6 siRNA samples. The clustered genes are listed in gray rectangles on the right side of the figure.