Figure 1

MiR-PC-86 and MiR-PC-263 directly regulate IGF-1R expression via 3’ UTR sites. (A) Schematic of IGF-1R mRNA and the luciferase reporter plasmids containing the miR-PC-86 and miR-PC-263 binding sites of IGF-1R mRNA. The 3’ UTR sites were inserted downstream of the luciferase reporter, as indicated. TCAGTGG was the predicted target site of miR-PC-86, GGATCTT was the predicted target site of miR-PC-263. (B) miR-PC-86 and miR-PC-263 sequences and predicted binding site between miR-PC-86 and miR-PC-263 and IGF-1R mRNA. IGF-1R mRNA has one putative binding site for miR-PC-86/ miR-PC-263 on the 3’ UTR. Twelve nucleotides TCAGTGGATCTT of IGF-1R 3’ UTR (underlined) were delete in order to disrupt the binding with miR-PC-86 and miR-PC-263 seed regions. (C) IPEC-J2 cells were transfected with each of the constructed plasmids, together with miR-PC-86/ miR-PC-263and Renilla luciferase reporter plasmid (*P < 0.05, n = 6).