Phylogenetic relationship of the Hsfs involving with Gm ( Glycine max ), At ( Arabidopsis thaliana ) Os ( Oryza sativa ), and Zm ( Zea mays ). The phylogenetic tree is produced by MEGA 5.0 software based on the comparison of amino acid sequences of the DNA binding domain, the HR-A/B region and the linker between these two regions. ScHsf1 was used as the out group. The neighbor-joining method was used and the bootstrap values were set at 1000. The frequency values (%) higher than 50 were showed nearby the branch lines. GmHsfs were divided into 3 classes and 12 subclasses (A1, A2, A3, A4, A5, A6, A8, B1, B2, B3, B4 and C1) and separated by red and blue branches. In this analysis, AtHsfA9 was classified into A8 subclasses.