Multiple sequence alignment of DNA binding domains of soybean Hsfs. DBD domain sequences of GmHsfs identified by Pfam database were aligned by Clustal X 2.0 software and edited by DNAMAN software. The black and gray backgrounds indicate entire conservative residues and 75% conservative residues respectively. The helix-turn-helix motifs of DBD (α1-β1-β2-α2-α3-β3-β4) are shown at the top. Cylindrical tubes represent α-helices and block arrows represent β-sheets.