Responses of GmHsf-34 overexpressing Arabdopsis to drought and heat stresses . (A) Seed germinations of wild-type (Col-0) and GmHsf-34 overexpressing Arabdopsis seeds. Seeds from three independent transgenetic lines of GmHsf-34 were grown on ½ MS medium with or without 4% PEG. Wild-type seeds were grown in the same condition as a control. Representative images of wild-type and one line (OE-1) of the transgenic plants were taken after treated by 4% PEG for 4 days. (B) Germination rates of wild-type and GmHsf-34 overexpressing Arabdopsis seeds. Germination rates were determined daily after 2 days stratification. Data represent means ± SD (n =90). (C) and (D) Root lengths of wild-type and GmHsf-34 overexpressing plants. Root length was measured by the ImageJ 2X software. Data represent means ± SD (n =90). (E) Representative images of wild-type and transgenic plants after treated under high temperature conditions. Four-week-old wild-type and GmHsf-34 overexpressing seedlings (OE-1, OE-2, and OE-3) were exposed to 42°C for 6 hours heat stress, and then transferred to normal condition. After one week, the photographs were taken. Three independent experiments were conducted. (F) Survival rates of wild-type and GmHsf-34 overexpressing plants after heat stress. One week after heat treated, survival rate of wild-type and transgenetic plants. Data represent means ± SD (n =90).