Figure 1

Sterigmatocystin production and expression analysis of A. nidulans ΔrsrA . (A) Thin Layer Chromatography analysis of chloroform extracts for metabolite production by the wild type (WT), ΔlaeA, ΔrsrA, ΔrsrAΔlaeA, ΔveA, and ΔveAΔrsrA, (RDIT9.32, RJW41A, RJW263.2 and RJW273.17, RJW112.2, and RJW113.4, respectively) strains grown on solid glucose minimal media (GMM) under light and dark at 37°C for 5 days in triplicate. ST, sterigmatocystin standard. *: 2.5 μL loading out of 100 μL sample. Others were loaded with 5 μL. (B) Gene expression analysis of A. nidulans strains, wild type (WT), ΔlaeA, ΔrsrA, ΔrsrAΔlaeA, ΔveA, and ΔveAΔrsrA grown on liquid GMM at 37°C, 225 rpm for 48 and 60 h in duplicate. Ethidium bromide-stained rRNA is indicated for loading.