Figure 2

CCL2, TNFα, and IFN-α concentration in lung homogenates of BXD recombinant inbred mouse strains. Forty-four different BXD RI mouse strains (derived from DBA/2J and C57BL/6) were infected with 10⁴ EID₅₀ of HK213 influenza virus in 30 μl PBS. Forty-eight hours post inoculation the lungs of the inoculated animals were collected, homogenized in sterile PBS, and stored at -80°C. The concentration of CCL2, TNFα, and IFN-α in these homogenates was quantified by ELISA. The average cytokine concentration (pg/ml ± standard error of the mean) of CCL2 (A), TNFα (B) and IFN-α (C) is shown for each BXD RI strain (black bars) and the parent strains (DBA/2J (white bar) and C57BL/6 (grey bar)). The average cytokine concentration for each BXD strain was calculated from 2-10 animals (average =5.2) and 36% of the BXD strains have been repeated independently.