Figure 3

Functional annotation analysis of differentially expressed genes in pancreatic islets of RasGrf1 KO mice. The DAVID functional annotation tool (http://david.abcc.ncifcrf.gov/) was used to identify statistically significant functional associations linking particular gene subsets contained within the list of repressed (blue) or induced (red) loci occurring in RasGrf1 KO pancreatic islets (Additional file 1: Table S1, FDR = 0.084) to specific Biological Process categories (Gene Ontology (GO) database, panel A) or Signaling Pathways (KEGG pathway database, panel B). Red: Induction. Blue: repression. Percentage values were calculated by referring the number of genes in each individual subset to the total number of repressed (1777) or induced (1943) genes recognized by DAVID within the list of differentially expressed genes listed in Table S1 (Additional file 1). The complete functional annotation analyses are described in Tables S2A,B (search against Biological Process GO database) (Additional files 3 and 4) and Tables S3A,B (search against KEGG signaling pathways database) (Additional files 5 and 6).