Figure 7

Quantitation of pancreatic islets and related cell lineages in RasGrf1 and Pttg1 KO mice. (A). Total pancreatic islet count in 8 month-old animals of the indicated genotypes. Count was performed under a dissecting microscope analyzing collagenase digests of total pancreas from WT, single KO and double KO mice at 8 months of age. Error bars indicate SD. * p < 0.05; ** p < 0.01; *** p < 0.005; n = 10 for WT; n = 11 for RasGrf1 KO; n = 6 for Pttg1 KO and n = 4 for RasGrf1/Pttg1 DKO. (B). Specific immunostaining of alpha (red) and beta (green) cells in pancreatic islets of WT and RasGrf1/Pttg1 DKO mice. Inmmunofluorescent staining for insulin (green) and glucagon (red) producing cells in paraformaldehyde-fixed pancreatic sections from WT and DKO mice (400 X). (C). Normalized numbers of alpha and beta cells in pancreatic islets of WT and RasGrf1/Pttg1 DKO mice. Numbers of alpha or beta cells per individual islet, as well as beta/alpha ratio in total pancreas are represented. The data documents a significantly higher reduction of the beta cell compartment in the DKO mice. Error bars indicate SD. * p < 0.05; ** p < 0.01; *** p < 0.005; n = 3.