Figure 5

The karyotype for C. aquatica HG mitotic metaphase chromosomes. The karyotype for C. aquatica HG mitotic metaphase chromosomes. (a) The mitotic metaphase chromosomes (numbered from 1 to 10 ) were counterstained with DAPI and pseudocolored in red; (b) FISH with the probe cocktail of SatS3 (green) and SatS2 (red); (c) A merged imagine with CentX in green and SatS2 in red; (d) The same spread was reprobed with the probe cocktail of SatS5 (green), 5S (red) and 45S (yellow); (e) The same spread was reprobed with the probe cocktails of SatS1 (green) and SatS2 (red); (f) The same spread was reprobed with the probe cocktail of CentX (green) and C. lacryma-jobi gDNA (red); (g) The twenty chromosomes closely homologous to C. lacryma-jobi were separated from figure (a-g) and listed in numerical order; (h) Ten chromosomes distant to C. lacryma-jobi were separated from figure (a-g) and listed in numerical order, bar = 5 μm.