Figure 3

CCA results using the Gata3 dataset for the Th differentiation programmes. CCA was applied to the Gata3 dataset, using the microarray dataset that analysed Th1, Th2, Th17, and iTreg (the Th dataset) as explanatory variables for the Th differentiation programmes. (a) Sample relationships in the first three axes. The Th differentiation programmes are shown by black lines (pink texts). (b) CCA triplot of Gata3-KO and WT samples (red, green, blue and cyan closed circles and squares), genes (grey closed circles), and the Th differentiation programmes (blue arrows). (c) Gene plot of the CCA solution in (a) and (b), showing Th-specific genes only. (d) CCA triplot using PCA gene scores (PC1-3) of the Th dataset as explanatory variables. (e, f) CCA sample scores using (e) Th2 and (f) Th1 differentiation variables.