Gene expression profiling by cDNA-AFLP reveals potential candidate genes for partial resistance of ‘Président Roulin’ against Venturia inaequalis

Table 1 Suitability of restriction enzymes for use in cDNA-AFLP analysis in apple

Enzyme^a	Restriction site	TDF visualized^b	N° cleavage sites	Position (bp)^c	Length (bp)^d
SAU3A	GATC	69%	5.5	723	133
TAQI	TCGA	67%	4.5	680	147
DDEI	CTNAG	70%	4.5	650	156
ECORII	CCWGG	66%	2.0	605	222
APOI	RAATTY	59%	2.4	676	167
ECORI	GAATTC	34%	0.5	683	234

^a450 Full Length cDNA from Malus x domestica were analyzed in silico for patterns of cleavage by different restriction enzymes in combination with MseI.
^bPercentage of cDNAs that yielded a TDF of a size that could be resolved on a 5% polyacrylamide gel (between 50 and 1000 bp) after cleavage with the particular enzyme in combination with MseI.
^cAverage distance between the last recognition site and the polyadenylation site.
^dMean size of restriction fragments, expressed in base pairs.

ISSN: 1471-2164