AGO1 targeting abilities of miR168 from corresponding species. (A) Target score for AGO1 targeting in different species by their miR168. TAPIR analysis was carried out as described in methods section. Abbreviation of plant names are given in Additional file 1: Table S1. (B) MFe ratios for the target/miR168 complementarity. Best targets have lower score and higher MFe ratio. (C) Predicted AGO1 targeting of miR168 from few representative species along with AGO1 from other species for comparison.