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Table 2 Gene expression confirmed using RT-qPCR under oxygen conditions (condition O2)

From: Early adaptation to oxygen is key to the industrially important traits of Lactococcus lactis ssp. cremoris during milk fermentation

     

Ratio of gene expression O2/N2 as determined by:

Locus tag

Gene

Gene product

Primer sequences

 

Transcriptome analysis

RT-qPCR

   

Forward

Reverse

1 h

5 h

8 h

1 h

5 h

8 h

llmg_0408

noxE

NADH oxidase

TTATGCCAAAGCAGAgGATTTT

GGAATAATTGGACGTGAACCTG

22.9

−3.2

 

26.7

2.0

 

llmg_0074

pdhA

pyruvate dehydrogenase E1 component alpha subunit

AGGACGTATGGGATTCTTTGG

TTACCAAGTTGATGTCCACGAG

6.0

−2.3

 

7.7

2.8

4.1

llmg_1541

nrdH

Glutaredoxin-like protein nrdH.

AATTGTATGCAATGCAAAATGG

ATTACAGGAGCAGCTCGAAAAC

10.3

 

2.8

28.3

4.7

6.9

llmg_0776

trxB2

thioredoxin reductase

TGGTCTtTATGCGGCTTTTTAt

GGTAAAGATTTTGtGGcTGACC

4.3

  

13.3

2.4

 

llmg_2432

adhE

alcohol-acetaldehyde dehydrogenase

GGTTCTGAAGTGACTCCATTTG

TCATAACAAACTCAGGGTCAACA

−7.5

  

−8.55

  

llmg_1464

adlC

alpha-acetolactate decarboxylase

GGCTGACCAACCTTATTTTACA

TTCaGTgATGAAATTTTGGACA

4.6

 

3.7

11.8

2.3

4.8

llmg_1699

choS

choline ABC transporter permease

TTTTGCAAGTCACaGGAATTTTT

GGaAAAATCGCATAAACAACAAG

3.6

  

10.0

4.1

3.1

lmg2144

lmg2144

conserved hypothetical protein

TtGGcGGAATaaTAGGctgt

cGTgCgtTgaAgAtAgAGACC

5.3

−4.3

 

9.0

 

2.7

lmg0274

lmg0274

conserved hypothetical protein

CTCggTCATcGGAAAAGAAG

TTtGTaGCTTgcTGCCAGAG

4.7

2.3

2.4

5.3

  

llmg_0100

cadA

cation-transporting ATPase

TTTGGCTTTAATCAGCCTTTTT

CCACCAGTAAAGTCGCAATAAT

−4.1

   

2.1

3.5

llmg_0349

fhuD

ferrichrome ABC transporter substrate binding protei

CATTAGGTGCAAATGTTGTTGG

TCAGGATTTTGAGCAATCAATTT

5.7

  

14.8

2.8

2.5

llmg_2050

tuf*

translation elongation factor EF-Tu

CACTCCATTCTTCGACAACTACC

AGGCATTACCATTTCAGTTCCTT

      

llmg_0102

parA*

chromosome partitioning protein parA.

TTcTACAgCCgATtATtGTTCGT

TGGgATtGTTTCTAAtCCAGCTA

      

llmg_0496

hllA*

HU-like DNA-binding protein

TTCAATTGATCGGTTTTGGTACT

TCAATGCTTTACCAGCTTTGAAT

      
  1. *tuf, parA and hllA expressions were used to normalised results.
  2. Fold-changes of differentially expressed genes, in O2 condition in comparison with N2 condition, confirmed using RT-qPCR with a p-value < 0.05 and a |fold-change| > 2 (n = 3). Genes parA, tuf and hllA were internal control genes.