RNA-seq and high-definition mass spectrometry reveal the complex and divergent venoms of two rear-fanged colubrid snakes

Table 4 Hypsiglena sp. LC/MS ^E protein identifications

Transcript	PLGS	Peptide	% Seq.
name	score	matches	coverage	Group
CRISP-1b	90,678.90	28	97.29	1
CRISP-1d	90,678.90	28	97.29	1
CRISP-1a	83,532.05	29	97.29	2
CRISP-1c	83,532.05	29	97.29	2
CRISP-1e	80,437.63	27	92.56	2
CTL-7e	36,677.55	17	96.24	3
CTL-7d	36,677.55	14	63.91	3
CTL-7b	36,677.55	17	96.24	3
CTL-7a	36,677.55	17	96.24	3
CTL-7c	36,564.01	15	84.96	3
CTL-8g	26,033.11	19	82.22	4
CTL-8f	25,919.58	18	81.62	4
CTL-8e	25,919.58	18	82.22	4
CTL-8d	25,919.58	18	81.62	4
CTL-8c	25,919.58	18	81.62	4
CTL-8b	25,919.58	18	81.62	4
CTL-8a	25,919.58	18	81.62	4
Fused	3,986.49	9	53.45	5
SVMPIII-1	2,196.46	23	41.14	6
SVMPIII-3a	6,757.38	33	44.41	7
SVMPIII-3b	6,757.38	34	44.41	7
SVMPIII-3c	6,754.13	33	44.41	7
SVMPIII-3d	6,754.65	32	44.41	7
SVMPIII-4	22,516.59	42	59.05	8
SVMPIII-5	5,473.71	36	43.56	9
SVMPIII-6a	4,589.49	27	39.20	10
SVMPIII-6b	4,596.38	29	39.20	10
SVMPIII-6c	4,589.49	27	39.20	10
SVMPIII-7a	10,796.01	28	61.87	11
SVMPIII-7b	10,792.75	27	61.87	11
SVMPIII-8a	3,825.85	26	28.45	12
SVMPIII-8b	3,825.85	26	28.45	12
SVMPIII-8c	3,940.07	29	26.94	12
SVMPIII-8d	3,855.83	27	28.45	12
SVMPIII-8e	3,848.95	25	28.45	12
SVMPIII-9a	22,893.75	38	59.32	13
SVMPIII-9b	22,893.75	38	59.32	13
SVMPIII-10a	1,531.99	23	27.04	14
SVMPIII-10b	1,531.99	23	27.04	14
SVMPIII-10c	1,531.99	23	27.04	14
SVMPIII-10d	1,525.10	22	27.04	14
SVMPIII-11a	6,924.50	33	45.29	15
SVMPIII-11b	6,893.95	32	42.91	15
SVMPIII-11c	6,901.40	33	43.60	15
SVMPIII-12a	9,702.24	42	56.54	16
SVMPIII-12b	9,702.24	42	56.54	16
SVMPIII-13a	16,451.10	38	54.15	17
SVMPIII-13b	16,460.75	39	54.15	17
SVMPIII-14a	4,436.73	34	39.86	18
SVMPIII-14b	4,436.73	34	38.56	18
SVMPIII-14c	4,442.71	37	39.86	18
Hemoglobin-b1	1,105.63	6	44.22	19
SVMPIII-2	5,775.72	40	39.80	–

Transcripts were grouped on the basis of shared, unique peptides. Identifications without a group designation lacked unique identifying peptides but still had peptide matches. Abbreviations: CTL–C-type lectin, CRISP–cysteine-rich secretory protein, SVMPIII–snake venom metalloproteinase (P-III).

ISSN: 1471-2164