Annotation for mutations in human cytoplasmic GRS. (A) Frequency distribution of residues at sites of GRS mutations. The distribution was calculated based on top 100 mammalian homologous sequences identified using BLAST. A ‘-’ represents frequency for gaps in the alignment. (B) Alignment between sequence and structure of human cytoplasmic GRS (UniProt ID P41250 and PDB ID 2ZT7) with mutations (boxes). Residues in GRS that interact with ATP/analogue and dimeric interface are underlined in black and blue respectively. Structural conservation for GRS is graded in four categories <40%, 40-60%, 60-80%, and >80% which are highlighted in magenta, blue, green and red respectively. (C) GRS mutations and those in dimeric interface are highlighted with a box.