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Table 1 Quantitative accuracy of the cleavable ICAT reagent in complex samples

From: Analysis of the early heterocyst Cys-proteome in the multicellular cyanobacterium Nostoc punctiforme reveals novel insights into the division of labor within diazotrophic filaments

Mixed ratio (light: heavy) 1:5 1:1 5:1
Expected 0.2 1.0 5.0
Average 0.168 0.882 5.41
Median 0.159 0.895 5.31
Stdev 0.088 0.142 1.24
95th percentile 0.227 1.093 7.73
5th percentile 0.103 0.657 3.63
Variance 0.008 0.020 1.53
Variance % 4.83 2.26 28.8
Number of proteins analyzed 146 213 150
  1. Relative abundance ratios of total proteins isolated from N2-fixing filaments of N. punctiforme labeled with the light- or heavy-ICAT reagent and mixed in 1:1, 1:5 and 5:1 ratios.