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Table 2 Comparative functional characterization of quantified proteins

From: Analysis of the early heterocyst Cys-proteome in the multicellular cyanobacterium Nostoc punctiforme reveals novel insights into the division of labor within diazotrophic filaments

   24 h (Present study) Steady-state[7]
Function   Total >Het % total Equal % total >Fil % total Total >Het % total Equal % total >Fil % total
Adaptive metabolism A 52 30 58 10 19 12 23 19 8 42 7 37 4 21
Core metabolism C 292 43 15 76 26 173 59 236 60 25 87 37 89 38
Transport metabolism T 16 5 31 9 56 2 13 13 7 54 5 38 1 8
Selfish metabolism S 1 0 0 0 0 1 100 0 0 0 0 0 0 0
Unassigned metabolism U 149 28 19 39 26 82 55 108 36 33 38 35 34 31
Not annotated NA 1 0 0 0 0 1 100 1 1 100 0 0 0 0
Total   511 106 21 134 26 271 53 377 112 30 137 36 128 34
Sub-function                
Adaptive metabolism                
 Carbon A 3 0 0 1 33 2 67 1 0 0 0 0 1 100
 Nitrogen A 14 13 93 0 0 1 7 5 3 60 2 40 0 0
 Development A 16 15 94 1 6 0 0 3 2 67 1 33 0 0
 Signal transduction A 7 1 14 2 29 4 57 4 2 50 1 25 1 25
 Stress A 2 1 50 1 50 0 0 2 0 0 0 0 2 100
 Taxis A 1 0 0 0 0 1 100 0 0 0 0 0 0 0
 Transcriptional regulation A 6 0 0 5 83 1 17 2 1 50 1 50 0 0
 Energy, monomer, polymer A 2 0 0 0 0 2 100 2 0 0 2 100 0 0
 Secondary A 1 0 0 0 0 1 100 0 0 0 0 0 0 0
Core metabolism                
 Precursor C 53 3 6 16 30 34 64 57 11 19 13 23 33 58
 Cofactor C 21 1 5 3 14 17 81 6 1 17 1 17 4 67
 Energy C 20 9 45 6 30 5 25 32 21 66 9 28 2 6
 Monomers C 54 5 9 4 7 45 83 23 1 4 10 43 12 52
 Polymers C 85 14 16 33 39 38 45 77 12 16 42 55 23 30
 Cell envelope C 29 11 38 5 17 13 45 20 6 30 6 30 8 40
 Cell division C 5 0 0 2 40 3 60 3 1 33 1 33 1 33
 Reactive oxygen species/metabolic protection C 15 0 0 4 27 11 73 15 7 47 3 20 5 33
 Storage C 10 0 0 3 30 7 70 3 0 0 2 67 1 33
Selfish metabolism                
 Phage S 1 0 0 0 0 1 100 0 0 0 0 0 0 0
Transport metabolism                
 ABC systems T 11 3 27 6 55 2 18 6 3 50 3 50 0 0
 Ion ATPase T 1 1 100 0 0 0 0 3 2 67 0 0 1 33
 Export T 3 0 0 3 100 0 0 4 2 50 2 50 0 0
 Porter T 1 1 100 0 0 0 0 0 0 0 0 0 0 0
Unassigned metabolism                
 Conserved hypothetical U 81 16 20 21 26 44 54 57 21 37 18 32 18 32
 Hypothetical U 11 1 9 1 9 9 82 5 1 20 2 40 2 40
 Unassigned U 57 11 19 17 30 29 51 46 14 30 18 39 14 30
Not annotated NA 1 0 0 0 0 1 100 1 1 100 0 0 0 0
  1. The relative abundance levels of proteins in isolated heterocyst vs. their parental N2-fixing filaments 24h (present study) and 6 days [7] after combined nitrogen step-down. The quantified proteins were grouped based on their functional annotation [27] and their relative differential abundance levels (using the Log2 +/-0.678 thresholds of 1.6-fold). >Het (higher in heterocysts); >Fil (higher in filaments, i.e. vegetative cells) and Equal (even distribution) indicate the individual proteins cell-type specificity using the thresholds defined in the two studies.