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Table 2 Genes with greater than 4-fold higher transcription during growth of R. albus 7 on cellulose compared to cellobiose as the sole carbohydrate source assessed by RNA-Seq

From: Unique aspects of fiber degradation by the ruminal ethanologen Ruminococcus albus 7 revealed by physiological and transcriptomic analysis

Gene (Rumal_)

Annotation1

Fold Change2

Signal sequence3

1715

Indole-3-glycerol-phosphate synthase

15.50

No

1714

chorismate mutase

15.49

No

1716

anthranilate phosphoribosyltransferase

14.90

No

1712

tryptophan synthase subunit alpha

14.90

No

1713

tryptophan synthase subunit beta

14.10

No

1717

anthranilate synthase component I

13.62

No

3687

transcriptional regulator, ArsR family

11.53

No

3686

protein of unknown function DUF1648

11.32

Sec

0360

helix-turn-helix domain-containing protein

10.04

No

3688

protein of unknown function UCP033101

9.98

TM

2652

putative multiple sugar transport system substrate-binding protein

9.80

Sec

2572

ECF subfamily RNA polymerase sigma-24 subunit

8.82

No

2573

hypothetical protein

8.18

TM

0897

fibronectin type III domain-containing protein (CBM37)

7.93

Sec

3780

Resolvase domain

7.91

No

2448

Cellulase (GH9 CBM3 CBM37 CBM37)

7.70

Sec

3766

hypothetical protein

7.42

Sec

0953

hypothetical protein

7.00

Sec

3796

ABC transporter family protein

5.91

No

1569

Cellulase (GH9 CBM37)

5.56

Sec

3775

ABC transporter family protein

5.53

No

3757

Endo-1,4-beta-xylanase (GH11 CBM22 GH10 CBM37)

5.51

Sec

3776

IstB domain protein ATP-binding protein

5.21

No

0896

Cellulase (GH5 CBM37)

5.15

Sec

1952

fibronectin type III domain-containing protein (PL1 CBM37)

5.00

Sec

3963

hypothetical protein

4.84

TM

1427

hypothetical protein

4.74

TM

0487

Ricin B lectin (CE12 CBM13 CBM35 CE12)

4.74

Sec

3179

Capsule synthesis protein CapA

4.68

No

2739

endo-1,4-beta-xylanase (GH11 CBM22 CE1 CBM37)

4.63

Sec

1262

fibronectin type III domain-containing protein (PL11 CBM37)

4.61

Sec

0908

endo-1,4-beta-xylanase (GH11 CBM22 CE4 CBM37)

4.59

Sec

3944

chaperone protein DnaK

4.48

No

1426

hypothetical protein

4.37

Sec

1821

family 1 extracellular solute-binding protein

4.36

Sec

3777

Integrase catalytic region

4.33

No

1428

hypothetical protein

4.30

TM

1602

transaldolase

4.26

No

2599

glycoside hydrolase family 5 (GH5 CBM37)

4.23

Sec

3940

hypothetical protein

4.22

No

3798

IS66 Orf2 family protein

4.22

No

3186

family 1 extracellular solute-binding protein

4.18

Sec

1951

fibronectin type III domain-containing protein (CBM37)

4.17

Sec

3184

binding-protein-dependent transport systems inner membrane component

4.16

TM

3185

binding-protein-dependent transport systems inner membrane component

4.12

No

3774

hypothetical protein

4.10

No

1044

spore coat protein CotH (CBM37)

4.09

Tat

3263

hypothetical protein

4.02

No

  1. 1Each gene reported here was significantly DE, with a PP of DE greater than 0.95
  2. 2Predicted signal sequence predicted by PRED-TAT. Sec = Sec-dependent; TM = transmembrane.
  3. 3Genes containing predicted CBM37 domains are presented in bold.