Validation of hormonal alteration in acutely exposed mice and zebrafish. (A) Vtg mRNA level measured by qRT-PCR in liver of zebrafish females (20 animals/group). Each sign is a single animal. (B) Serum estradiol levels measured by ELISA assay in acute exposed mouse females (15 animals/group). Each sign is a single animal. Mean and standard deviation is reported. *p-value ≤0,05, **p-value ≤0,01.